A GLOBAL APPROACH FOR QUANTITATIVE SUPER RESOLUTION AND ELECTRON MICROSCOPY ON CRYO AND EPOXY SECTIONS USING SELF-LABELING PROTEIN TAGS

A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags

Abstract Correlative light and electron microscopy (CLEM) is fleshlight automatique a powerful approach to investigate the molecular ultrastructure of labeled cell compartments.However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to strong fixatives and contrasting reagents for EM

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In planta transcriptomics reveals conflicts between pattern-triggered immunity and the AlgU sigma factor regulon.

In previous work, we determined the transcriptomic impacts of flg22 pre-induced Pattern Triggered Immunity (PTI) in Arabidopsis thaliana on the pathogen Pseudomonas syringae pv.tomato DC3000 (Pto).During PTI exposure we observed expression patterns in Pto reminiscent of those previously observed in a Pto algU mutant.AlgU is a conserved extracytopla

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